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1. Introduction

Locating and Correcting the Problem

A systematic approach is best to identify any problems when troubleshooting the high-performance liquid chromatography (HPLC) system. This guide is organized into five major categories of symptoms to help quickly identify the source of the problem(s).

Pressure abnormalities
Leaks
Problems with the chromatogram
Injector problems
Other problems detected by smell, sight, and sound

When the problem has been corrected, record the incident in the HPLC system record book to help with future problems.

Prevention

Many liquid chromatography (LC) problems can be prevented with routine maintenance. For example, replacing pump seals at regular intervals should eliminate pump-seal failure and its associated problems. Section 7 lists the most common problem areas for each LC module, as well as preventive maintenance practices to reduce their frequency. These suggestions can be modified to fit any particular model of LC, and should be made a regular part of the laboratory routine.

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