Figure 8. Sensorgrams depicting the on-chip purification and biosensor-binding assay. (A) Overlay of a complete series of real-time sensorgrams, which represent (from left) injections of cell-free media containing sIL5Rα; (B) injection of running buffer; (C) injections of 0, 5, 10, 20, and 40 nm of IL5 followed by running buffer alone; and (D) injections of the regeneration buffer.