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Chapter 2: One–dimensional Polyacrylamide Gel Electrophoresis—References

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Atkins R.E., Levin P.M., and Taun R.S. 1992. Cetyltrimethylammonium bromide discontinuous gel electrophoresis: Mr-based separation of proteins with retention of enzymatic activity. Anal. Biochem. 202: 172–178.

Bollag D.M, Rozycki M.D., and Edelstein S.J., eds. 1996. Protein methods, 2nd edition. Wiley, New York.

Caglio S., Chiari M., and Righetti P.G. 1994. Gel polymerization in detergents: Conversion efficiency of methylene blue vs. persulfate catalysis, as investigated by capillary zone electrophoresis. Electrophoresis 15: 209–214.

Cantor C.R. and Schimmel P.R. 1980. Biophysical chemistry. II. Techniques for the study of biological structure and function, pp. 676–680. W.H. Freeman, San Francisco.

Chrambach A. and Rodbard D. 1971. Polyacrylamide gel electrophoresis. Science 172: 440–451.

Chrambach A. and Jovin T.M. 1983. Selected buffer system for moving boundary electrophoresis on gels at various pH values, presented in a simplified manner. Electrophoresis 4: 190–204.

Davis B.J. 1964. Disc electrophoresis. II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121: 401–349.

de Moreno M.R., Smith J.F., and Smith R.V. 1986. Mechanism studies of Coomassie blue and silver staining of proteins. J. Pharm. Sci. 75: 907–911.

Duke J.A., Bier M., and Nord F.F. 1952. On the mechanism of enzyme action. LIII. The amphoteric properties of trypsin. Arch. Biochem. Biophys. 40: 424–436.

Fazekas de St. Groth S., Webster R.G., and Datyner A. 1963. Two new staining procedures for quantitative estimation of proteins on electrophoretic strips. Biochim. Biophys. Acta 71: 377–391.

Ferguson K.A. 1964. Starch-gel electrophoresis: Application to the classification of pituitary proteins and polypeptides. Metabl. Clin. Exp. 13: 985–1002.

Gersten D.M. and Bijwaard K.E. 1992a. Separation of proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of soluble, aqueous polymers: Ficoll and polyvinylpyrrolidone. Electrophoresis 13: 399–401.

_______. 1992b. Polyacrylamide gel electrophoresis in vertical, inverse and double-crossing gradients of soluble polymers. Electrophoresis 13: 282–286.

Granzier H.L.M. and Wang K. 1993. Gel electrophoresis of giant proteins: Solubilization and silver-staining of titin and nebulin from single muscle fiber segments. Electrophoresis 14: 56–64.

Hames B.D., ed. 1998. Gel electrophoresis of proteins: A practical approach, 3rd edition. Oxford University Press, New York.

Hames B.D. 1990. One-dimensional polyacrylamide gel electrophoresis. In Gel electrophoresis of proteins: A practical approach, 2nd edition (ed. B.D. Hames and D. Rickwood). Oxford University Press, New York.

Harwig S.S., Chen N.P., Park A.S., and Lehrer R.I. 1993. Purification of cysteine-rich bioactive peptides from leukocytes by continuous acid-urea-polyacrylamide gel electrophoresis. Anal. Biochem. 208: 382–386.

Hjertén S. 1962. "Molecular Sieve" chromatography on polyacrylamide gels, prepared according to a simplified method. Arch. Biochem. Biophys. (suppl. 1) 147–151.

Kaufmann E., Geisler N., and Weber K. 1984. SDS-PAGE strongly overestimates the molecular masses of the neurofilament proteins. FEBS Lett. 170: 81–84.

Kohlrausch F. 1897. Ueber concentrations-verschiebungen durch elecrolyse im innern von lösungen und lösungsgemischen. Ann. Phys. Chem. 62: 210–239.

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MacFarlane D.E. 1989. Two dimensional benzyldimethyl-n-hexadecylammonium chloride–sodium dodecyl sulfate preparative polyacrylamide gel electrophoresis: A high capacity high resolution technique for the purification of proteins from complex mixtures. Anal. Biochem. 176: 457–463.

Makowski G.S. and Ramsby M.L. 1993. pH modification to enhance the molecular sieving properties of sodium dodecyl sulfate-10% polyacrylamide gels. Anal. Biochem. 212: 283–285.

_______. 1997. Protein molecular weight determination by sodium dodecyl sulfate polyacrylamide gel electrophoresis. In Protein structure: A practical approach, 2nd edition (ed. T.E. Creighton), pp. 1–27. IRL Press/Oxford University Press, New York.

McLellan T. 1982. Electrophoresis buffers for polyacrylamide gels at various pH. Anal. Biochem. 126: 94–99.

Meyer T.S. and Lamberts B.L. 1965. Use of Coomassie brilliant blue R250 for the electrophoresis of microgram quantities of parotid saliva proteins on acrylamide gel strips. Biochim. Biophys. Acta 107: 144–145.

Mócz G. and Bálint M. 1984. Use of cationic detergents for polyacrylamide gel electrophoresis in multiphasic buffer systems. Anal. Biochem. 143: 283–292.

Morris C.J.O.R. 1966. Gel filtration and gel electrophoresis. In Protides of the biological fluids (ed. H. Peeters), vol 14, pp. 543–561. Elsevier, Amsterdam.

Neuhoff V., Arold N., Taube D., and Ehrhardt W. 1988. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9: 255–262.

Neuhoff V., Stamm R., Pardowitz I., Arold N., Ehrhardt W., and Taube D. 1990. Essential problems in quantification of proteins following staining with Coomassie Brilliant Blue dyes in polyacrylamide gels, and their solution. Electrophoresis 11: 101–117.

Neville D.M. Jr. 1971. Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. J. Biol. Chem. 246: 6328–6334.

Ornstein L. 1964. Disc electrophoresis. I. Background and theory. Ann. N.Y. Acad. Sci. 121: 321–349.

Panyim S. and Chalkley R. 1969. High resolution acrylamide gel electrophoresis of histones. Arch. Biochem. Biophys. 130: 337–346.

Patton W.F. 2000. A thousand points of light: The application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics. Electrophoresis 21: 1123–1144.

_______. 2001. Detecting proteins in polyacrylamide gels and on electroblot membranes. In Proteomics: From protein sequence to function (ed. S.R. Pennington and M.J. Dunn), pp. 65–86. BIOS Scientific, Oxford, United Kingdom, Springer-Verlag, New York.

Rabilloud T. 2000. Detecting proteins separated by 2-D gel electrophoresis. Anal. Chem. 72: 48A–55A.

Rabilloud T., Girardot V., and Lawrence J.J. 1996. One- and two-dimensional histone separations in acidic gels: Usefulness of methylene blue-driven photopolymerization. Electrophoresis 17: 67–73.

Raymond S. and Weintraub L.S. 1959. Acrylamide gel as a supporting medium for zone electrophoresis. Science 130: 711.

Reynolds J.A. and Tanford C. 1970. Binding of dodecyl sulfate to proteins at high binding ratios. Possible implications for the state of proteins in biological membranes. Proc. Natl. Acad. Sci. 66: 1002–1007.

Righetti P.G. 1995. Macroporous gels: Facts and misfacts. J. Chromatogr. A698: 3–17.

Schägger H. and von Jagow G.V. 1987. Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166: 368–379.

Segrest J.P. and Jackson R.L. 1972. Molecular weight determination of glycoproteins by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Methods Enzymol. 28B: 54–63.

Shapiro A.L., Viñuela E., and Maizel J.V. 1967. Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem. Biophys. Res. Commun. 28: 815–820.

Shi Q. and Jackowski G. 1998. One-dimensional polyacrylamide gel electrophoresis. In Gel electrophoresis of proteins: A practical approach, 3rd edition (ed. B.D. Hames), pp. 1–52. IRL Press/Oxford University Press, NewYork.

Sinha P., Poland J., Schnolzer M., and Rabilloud T. 2001. A new silver staining apparatus and procedure for matrix-assisted laser desorption/ionization-time of flight analysis of proteins after two-dimensional electrophoresis. Proteomics 1: 835–840.

Smith B.J. 1994. SDS polyacrylamide gel electrophoresis of proteins. Methods Mol. Biol. 32: 23–34.

Sobieszek A. 1994. Gradient polyacrylamide gel electrophoresis in presence of sodium dodecyl sulfate: A practical approach to muscle contractile and regulatory proteins. Electrophoresis 15: 1014–1020.

Swank R.T. and Munkres K.D. 1971. Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate. Anal. Biochem. 39: 462–477.

Weber K. and Osborn M. 1969. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244: 4406–4412.

Wong C., Sridhara S., Bardwell J.C.A., and Jakob U. 2000. Heating greatly speeds Coomassie blue staining and destaining. BioTechniques 28: 426–432.

Further Reading

Righetti P.G., Faupal M., and Wenisch E. 1992. Preparative electrophoresis with and without immobilized pH gradients. In Advances in electrophoresis (ed. A. Chrambach et al.), vol. 5, pp. 159–200. VCH, Weinheim, Germany.

 
 
 

 
   
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