.
. Proteins and Proteomics: A Laboratory ManualCSHL Press .
. . . . .
.
.
.
. .
 
.
. . .
.  FIRST VISIT?
 TRY THESE LINKS
.
. . .
.
.   Enroll for Updates
  Privacy Policy
  Purchase the book
.
.
 
 
.
. . .
.  BOOK COVER .
. . .
.
. Proteins and Proteomics: A Laboratory Manual cover .
.
CLICK TO ENLARGE
 
Buy the Book
 
   
 

Chapter 4: Preparative Two–dimensional Gel Electrophoresis with Immobilized pH Gradients—References

Adessi C., Miege C., Albrieux C., and Rabilloud T. 1997. Two-dimensional electrophoresis of membrane proteins: A current challenge for immobilized pH gradients. Electrophoresis 18: 127–135.

Bjellqvist B., Ek K., Righetti P.G., Gianazza E., Görg A., Westermeier R., and Postel W. 1982. Isoelectric focusing in immobilized pH gradients: Principle, methodology, and some applications. J. Biochem. Biophys. Methods 6: 317–339.

Bjellqvist B., Sanchez J.C., Pasquali C., Ravier F., Paquet N., Frutiger S., Hughes G.J., and Hochstrasser D. 1993. Micropreparative two-dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins. Electrophoresis 14: 1375–1378.

Blomberg A., Blomberg L., Norbeck J., Fey S.J., Larsen P.M., Larsen M., Roepstorff P., Degand H., Boutry M., Posch A., and Görg A. 1995. Interlaboratory reproducibility of yeast protein patterns analyzed by immobilized pH gradient two-dimensional gel electrophoresis. Electrophoresis 16: 1935–1945.

Chevallet M., Santoni V., Poinas A., Rouquie D., Fuchs A., Kieffer S., Rossignol M., Lunardi J., Garin J., and Rabilloud T. 1998. New zwitterionic detergents improve the analysis of membrane proteins by two-dimensional electrophoresis. Electrophoresis 19: 1901–1909.

Corthals G.L., Molloy M.P., Herbert B.R., Williams K.L., and Gooley A.A. 1997. Prefractionation of protein samples prior to two-dimensional electrophoresis. Electrophoresis 18: 317–323.

Damerval C., de Vienne D., Zivy M., and Thiellement H. 1986. Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins. Electrophoresis 7: 52–54.

Dunbar B.S. 1987. Two-dimensional electrophoresis and immunological techniques. Plenum Press, New York.

Flengsrud R. and Kobro G. 1989. A method for two-dimensional electrophoresis of proteins from green plant tissues. Anal. Biochem. 177: 33–36.

Görg A., Postel W., and Günther S. 1985. Improved horizontal two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 6: 599–604.

_______. 1988. The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 9: 531–546.

Gatlin C.L., Kleemann G.R., Hays L.G., Link A.J., and Yates III J.R. 1998. Protein identification at the low femtomole level from silver-stained gels using a new fritless electrospray interface for liquid chromatography-microspray and nanospray mass spectrometry. Anal. Biochem. 263: 93–101.

Gharahdaghi F., Weinberg C.R., Meagher D.A., Imai B.S., and Mische S.M. 1999. Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: A method for the removal of silver ions to enhance sensitivity. Electrophoresis 20: 601–605.

Gianazza E. 1999. Casting immobilized pH gradients (IPGs). Methods Mol. Biol. 112: 175–188.

Görg A., Obermaier C., Boguth G., Harder A., Scheibe B., Wildgruber R., and Weiss W. 2000. The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 21: 1037–1053.

Guy G.R., Philip R., and Tan Y.H. 1994. Analysis of cellular phosphoproteins by two-dimensional gel electrophoresis: Applications for cell signaling in normal and cancer cells. Electrophoresis 15: 417–440.

Hancock K. and Tsang V.C.M. 1983. India ink staining of proteins on nitrocellulose paper. Anal. Biochem. 133: 157–162.

Herbert B.R., Molloy M.P., Gooley A.A., Walsh B.J., Bryson W.G., and Williams K.L. 1998. Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent. Electrophoresis 19: 845–851.

Hochstrasser D.F. and Merril C.R. 1988. Catalysts for polyacrylamide gel polymerization and detection of proteins by silver staining. Appl. Theor. Electrophoresis 1: 35–40.

Jensen O.N., Mortensen P., Vorm O., and Mann M. 1997. Automation of matrix-assisted laser desorption/ionization mass spectrometry using fuzzy logic feedback control. Anal. Chem. 69: 1706–1714.

Ji H., Whitehead R.H., Reid G.E., Moritz R.L., Ward L.D., and Simpson R.J. 1994. Two-dimensional electrophoretic analysis of proteins expressed by normal and cancerous human crypts: Application of mass spectrometry to peptide-mass fingerprinting. Electrophoresis 15: 391–405.

Ji H., Reid G.E., Moritz R.L., Eddes J.S., Burgess A.W., and Simpson R.J. 1997. A two-dimensional gel database of human colon carcinoma proteins. Electrophoresis 18: 605–613.

Klose J. 1975. Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals. Humangenetik 26: 231–243.

Laemmli U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.

Lee K.H. and Harrington M.G. 1999. Double-label analysis. Methods Mol. Biol. 112: 291–298.

Lenstra J.A. and Bloemendal H. 1983. Topography of the total protein population from cultured cells upon fractionation by chemical extractions. Eur. J. Biochem. 135: 413–423.

Link A.J. 1999. Autoradiography of 2-D gels. Methods Mol. Biol. 112: 289–290.

Lopez M.F., Berggren K., Chernokalskaya E., Lazarev A., Robinson M., and Patton W. 2000. A comparison of silver stain and SYPRO ruby protein gel stain with respect to protein detection in two-dimensional gels and identification by peptide mass profiling. Electrophoresis 21: 3673–3683.

Matsudaira P. 1987. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J. Biol. Chem. 262: 10035–10038.

Merrill C.R. and Harrington M.G. 1989. Silver stain protein detection: Mechanisms and applications. In Two-dimensional electrophoresis: Proceedings of the International Two-dimensional Electrophoresis Conference, Vienna, November 1988 (ed. A.T Endler and S. Hanash), pp. 243– 255. VCH, Weinheim, Germany.

Moeremans M., Daneels G., and De Mey J. 1985. Sensitive colloidal metal (gold or silver) staining of protein blots on nitrocellulose membranes. Anal. Biochem. 145: 315–321.

Molloy M.P., Herbert B.R., Walsh B.J., Tyler M.I., Traini M., Sanchez J.-C., Hochstrasser D.F., Williams K.L., and Gooley A.A. 1998. Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis 19: 837–844.

Musante L., Candiano G., and Ghiggeri G.M. 1997. Resolution of fibronectin and other uncharacterized proteins by two-dimensional polyacrylamide electrophoresis with thiourea. J. Chromatogr. 705: 351–356.

Neuhoff V., Arold N., Taube N., and Ehrhardt W. 1988. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9: 255–262.

O'Farrell P.H. 1975. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250: 4007–4021.

Patton W. 1995. Biologist's perspective on analytical imaging systems as applied to protein gel electrophoresis. J. Chromatogr. A 698: 55–87.

_______. 2000. A thousand points of light: The application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics. Electrophoresis 21: 1123–1144.

Patton W.F., Lim M.J., and Shepro D. 1999. Image acquisition in 2-D electrophoresis. Methods Mol. Biol. 112: 353–362.

Perdew G.H., Schaup H.W., and Selivonchick D.P. 1983. The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes. Anal. Biochem. 135: 453–455.

Rabilloud T., Valette C., and Lawrence J.J. 1994a. Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension. Electrophoresis 15: 1552–1558.

Rabilloud T., Adessi C., Giraudel A., and Lunardi J. 1997. Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 18: 307–316.

Rabilloud T., Gianazza E., Cattò N., and Righetti P.G. 1990. Amidosulfobetaines, a family of detergents with improved solubilization properties: Application for isoelectric focusing under denaturing conditions. Anal. Biochem. 185: 94–102.

Rabilloud T., Vuillard L., Gilly C., and Lawrence J.J. 1994b. Silver staining of proteins in polyacrylamide gels: A general overview. Cell. Mol. Biol. 40: 57–75.

Raman B., Cheung A., and Marten M.R. 2002. Quantitative comparison and evaluation of two commercially available, two-dimensional electrophoresis image analysis software packages, Z3 and Melanie. Electrophoresis 23: 2194–2202.

Ramsby M.L., Makowski G.S., and Khairallah E.A. 1994. Differential detergent fractionation of isolated hepatocytes: Biochemical, immunochemical and two-dimensional gel electrophoresis characterization of cytoskeletal and noncytoskeletal compartments. Electrophoresis 15: 265–277.

Salinovich O. and Montelano R.C. 1986. Reversible staining and peptide mapping of proteins transferred to nitrocellulose after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal. Biochem. 156: 341–351.

Sanchez J.C., Rouge V., Pisteur M., Ravier F., Tonella L., Moosmayer M., Wilkins M.R., and Hochstrasser D.F. 1997. Improved and simplified in-gel sample application using reswelling of dry immobilized pH gradients. Electrophoresis 18: 324–327.

Santoni V., Rabilloud T., Doumas P., Rouquié D., Mansion M., Kieffer S., Garin J., and Rossignol M. 2000. Towards the recovery of hydrophobic proteins on two-dimensional electrophoresis gels. Electrophoresis 20: 705–711.

Schägger H. and von Jagow G. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166: 368–379.

Scheler C., Lamer S., Pan Z., Li X., Salnikow J., and Jungblut P. 1998. Peptide mass fingerprint sequence coverage from differently stained proteins on two-dimensional electrophoresis patterns by matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS). Electrophoresis 19: 918–927.

Shevchenko A., Wilm M., Vorm O., and Mann M. 1996. Mass spectrometric sequencing of protein silver-stained polyacrylamide gels. Anal. Chem. 68: 850–858.

Sinha P., Poland J., Schnölzer M., and Rabilloud T. 2001. A new silver staining apparatus and procedure for matrix/ionization-time of flight analysis of proteins after two-dimensional electrophoresis. Proteomics 1: 835–840.

Sutherland J. 1993. Electronic imaging of electrophoretic gels and blots. Adv. Electrophor. 6: 1–42.

Switzer R.C., Merril C.R., and Shifrin S. 1979. A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal. Biochem. 98: 321–327.

Taylor R.S., Wu C.C., Hays L.G., Eng J.K., Yates J.R.I., and Howell K.E. 2000. Proteomics of rat liver Golgi complex: Minor proteins are identified through sequential fractionation. Electrophoresis 21: 3441–3459.

Towbin H., Staehelin T., and Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedures and some applications. Proc. Natl. Acad. Sci. 76: 4350–4354.

Tsugita A., Kamo M., Kawakami T., and Ohki Y. 1996. Two-dimensional electrophoresis of plant proteins and standardization of gel patterns. Electrophoresis 17: 855–865.

Usuda H. and Shimogawara K. 1995. Phosphate deficiency in maize. 6. Changes in the two-dimensional electrophoretic patterns of soluble proteins from second leaf blades associated with induced senescence. Plant Cell Physiol. 36: 1149–1155.

Weber K. and Osborne M. 1969. The reliability of molecular weight determination by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244: 4406–4412.

Yan J.X., Harry R.A., Spibey C., and Dunn M.J. 2000a. Postelectrophoresis staining of proteins by two-dimensional gel electrophoresis using SYPRO dyes. Electrophoresis 21: 3657–3665.

Yan J.X., Walt R., Berkelman T., Harry R.A., Westbrook J.A., Wheeler C.H., and Dunn M.J. 2000b. A modified silver staining protocol for visualization of proteins compatible with matix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry. Electrophoresis 21: 3666–3672.

 
 
 

 
   
. .