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Chapter 5: Reversed–phase High–performance Liquid Chromatography—References

Aguilar M.I., Hodder A.N., and Hearn M.T.W. 1985. High-performance liquid chromatography of amino acids, peptides and proteins. LXV. Studies on the optimization of the reversed-phase gradient elution of polypeptides: Evaluation of retention relationships with beta-endorphin related polypeptides. J. Chromatogr. 327: 115–138.

Alvarez V.L., Roitsch C.A., and Henriksen O. 1981. Purification of H-2a heavy chain and beta-microglobulin by reversed-phase high-performance liquid chromatography. Anal. Biochem. 115: 353–358.

Amersham Pharmacia Biotech. 2002. Reversed phase chromatography: Principles and methods, publication no. 18-1134-16. Amersham Biosciences, Buckinghamshire, United Kingdom.

Bennett H.P. 1983. Isolation of pituitary peptides by reversed-phase high performance liquid chromatography. Expansion of the resolving power of reversed-phase columns by manipulating pH and the nature of the ion-pairing reagent. J. Chromatogr. 266: 501–510.

_______. 1991. Manipulation of pH and ion-pairing reagents to maximize the performance of reversed-phase columns. In High-performance liquid chromatography of peptides and proteins: Separation, analysis, and conformation (ed. C.T. Mant and R.S. Hodges), pp. 319–326. CRC Press, Boca Raton, Florida.

Berchtold M.W., Wilson K.J., and Heizmann C.W. 1982. Isolation of neuronal parvalbumin by high-performance liquid chromatography. Characterization and comparison with muscle parvalbumin. Biochemistry 21: 6552–6557.

Berridge J.C. 1986. Techniques for the automated optimization of HPLC separations. Wiley Interscience, Chichester, United Kingdom.

Boysen R.I. and Hearn M.T.W. 2000. Direct characterisation by electrospray ionisation mass spectroscopy of mercuro-polypeptide complexes after deprotection of acetamidomethyl groups from protected cysteine residues of synthetic polypeptides. J. Biochem. Biophys. Methods 45: 157–168.

Boysen R.I., Erdmann V.A., and Hearn M.T.W. 1998. Systematic, computer-assisted optimisation of the isolation of Thermus Thermophilus 50S ribosomal proteins by reversed-phase high-performance liquid chromatography. J. Biochem. Biophys. Methods 37: 69–89.

Burgess A.W., Knesel J., Sparrow L.G., Nicola N.A., and Nice E.C. 1982. Two forms of murine epidermal growth factor: Rapid separation by using reversed-phase HPLC. Proc. Natl. Acad. Sci. 79: 5753–5757.

Chervet J.P., Ursem M., Salzmann J.P., and Vannort R.W. 1989. Ultra-sensitive UV detection in micro separation. HRC J. High Resolut. Chromatogr. 12: 278–281.

Chesterman C.N., Walker T., Grego B., Chamberlain K., Hearn M.T., and Morgan F.J. 1983. Comparison of platelet-derived growth factor prepared from release products of fresh platelets and from outdated platelet concentrates. Biochem. Biophys. Res. Commun. 116: 809–816.

Cohen K.A., Schellenberg K., Benedek K., Karger B.L., Grego B., and Hearn M.T. 1984. Mobile-phase and temperature effects in the reversed phase chromatographic separation of proteins. Anal. Biochem. 140: 223–235.

Cooke N.H.C., Archer B.G., O'Hare M.J., Nice E.C., and Capp M. 1983. Effects of chain length and carbon load on the performance of alkyl-bonded silicas for protein separations. J. Chromatogr. 255: 115–123.

Davis M.T. and Lee T.D. 1992. Analysis of peptide mixtures by capillary high performance liquid chromatography: A practical guide to small-scale separations. Protein Sci. 1: 935–944.

Dolan J.W. 1991. Preventive maintenance and troubleshooting LC instrumentation. In High-performance liquid chromatography of peptides and proteins: Separation, analysis, and conformation (ed. C.T. Mant and R.S. Hodges), pp. 23–29. CRC Press, Boca Raton, Florida.

Dolan J.W., Lommen D.C., and Snyder L.R. 1989. DryLab computer simulation for high-performance liquid chromatographic method development. II. Gradient elution. J. Chromatogr. 485: 91–112.

Forage R.G., Ring J.M., Brown R.W., McInerney B.V., Cobon G.S., Gregson P., Robertson D.M., Morgan F.J., Hearn M.T.W., Findlay J.K., Wettenhall R.E.H., Burger H.G., and de Kretser D.M. 1986. Cloning and sequence analysis of cDNA species coding for the two subunits of inhibin from bovine follicular fluid. Proc. Natl. Acad. Sci. 83: 3091–3095.

Gerber G.E., Anderegg R.J., Herlihy W.C., Gray C.P., Biemann K., and Khorana H.G. 1979. Partial primary structure of bacteriorhodopsin: Sequencing methods for membrane proteins. Proc. Natl. Acad. Sci. 76: 227–231.

Ghrist B.F.D. and Snyder L.R. 1988a. Design of optimized high-performance liquid chromatographic gradients for the separation of either small or large molecules. II. Background and theory. J. Chromatogr. 459: 25–41.

_______. 1988b. Design of optimized high-performance liquid chromatographic gradients for the separation of either small or large molecules. III. An overall strategy and its application to several examples. J. Chromatogr. 459: 43–63.

Ghrist B.F.D., Coopermann B.S., and Snyder L.R. 1988. Design of optimized high-performance liquid chromatographic gradients for the separation of either small or large molecules. Minimizing errors in computer simulations. J. Chromatogr. 459: 1–23.

Glajch J.L., Quarry M.A., Vasta J.F., and Snyder L.R. 1986. Separation of peptide mixtures by reversed-phase gradient elution. Use of flow rate changes for controlling band spacing and improving resolution. Anal. Chem. 58: 280–285.

Grego B. and Hearn M.T. 1984. High-performance liquid chromatography of amino acids, peptides and proteins. LXIII. Reversed-phase high-performance liquid chromatographic characterisation of several polypeptide and protein hormones. J. Chromatogr. 336: 25–40.

Grego B., Baldwin G.S., Knessel J.A., Simpson R.J., Morgan F.J., and Hearn M.T. 1984. High-performance liquid chromatography of amino acids, peptides and proteins. LVIII. Application of reversed-phase high-performance liquid chromatography to the separation of tyrosine-specific phosphorylated polypeptides related to human growth hormone. J. Chromatogr. 297: 21–29.

Gurley L.R., D'Anna J.A., Blumenfeld M., Valdez J.G., Sebring R.J., Donahue P.R., Prentice D.A., and Spall W.D. 1984. Preparation of histone variants and high-mobility group proteins by reversed-phase high-performance liquid chromatography. J. Chromatogr. 297: 147–165.

Hancock W.S., ed. 1984. CRC handbook of HPLC for the separation of amino acids, peptides and proteins. CRC Press, Boca Raton, Florida.

Hancock W.S. and Sparrow J.T. 1983. The separation of proteins by reversed-phase high-performance liquid chromatography. In High-performance liquid chromatography. Advances and perspectives (ed. C. Horváth), vol. 3, pp. 50–87. Academic Press, New York.

Hancock W.S., Bishop C.A., Prestige R.L., Harding D.R.K., and Hearn M.T.W. 1978a. Reversed-phase high-pressure liquid chromatography of peptides and proteins with ion-pairing ragents. Science 200: 1168–1170.

Hancock W.S., Bishop C.A., Prestige R.L., Harding D.R.K., and Hearn M.T.W. 1978b. High-pressure liquid chromatography of peptides and proteins. II. The use of phosphoric acid in the analysis of underivatized peptides by reversed-phase high-pressure liquid chromatography. J. Chromatogr. 153: 391398.

Hancock W.S., Bishop C.A., Gotto A.M., Harding D.R., Lamplugh S.M., and Sparrow J.T. 1981. Separation of the apoprotein components of human very low density lipoproteins by ion-paired, reversed-phase high performance liquid chromatography. Lipids 16: 250–259.

Hearn M.T.W. 1980. Ion-pair chromatography on normal and reversed-phase systems. Adv. Chromatogr. 18: 59–100.

_______. 1984. Reversed-phase high performance liquid chromatography of proteins. Methods Enzymol. 104: 190–212.

_______. 1985. Ion-pair chromatography of amino acids, peptides, and proteins. In Ion-pair chromatography: Theory and biological and pharmaceutical applications (ed. M.T.W. Hearn), pp. 207–257. Marcel Dekker, New York.

_______. 1991a. Current status and future challenges of high performance liquid chromatographic techniques for biopolymer analysis and purification. In HPLC of peptides, proteins and polynucleotides (ed. M.T.W. Hearn), pp. 1–35. VCH, New York.

_______. 1991b. High-performance liquid chromatography of peptides and proteins: General principles and basic theory. In High-performance liquid chromatography of peptides and proteins: Separation, analysis, and conformation (ed. C.T. Mant and R.S. Hodges), pp. 95–104. CRC Press, Boca Raton, Florida.

_______. 1991c. High-performance liquid chromatography of peptides and proteins: Quantitative relationships for isocratic and gradient elution of peptides and proteins. In High-performance liquid chromatography of peptides and proteins: Separation, analysis, and conformation (ed. C.T. Mant and R.S. Hodges), pp. 105–122. CRC Press, Boca Raton, Florida.

_______. 1998. High-resolution reversed-phase chromatography of polypeptides and proteins. In Protein purification: Principles, high-resolution methods, and applications, 2nd edition. (ed. J.-C. Janson and L. Rydén), pp. 239–282. Wiley-Liss, New York.

_______. 2000a. Conformational behaviour of polypeptides and proteins in reversed-phase environments. In Theory and practice of biochromatography (ed. M.A. Vijayalakshmi), pp. 72–235. Harwood Academic Publishers, Switzerland.

_______. 2000b. Physicochemical factors in polypeptide and protein purification and analysis by high-performance liquid chromatographic techniques: Current status and future challenges. In Handbook of bioseparation (ed. S. Ahyja), pp. 72–235. Academic Press, San Diego, California.

_______. 2002. Reversed-phase and hydrophobic interaction chromatography of proteins and peptides. In HPLC of biological macromolecules (ed. K.M. Gooding and F.E. Regnier), pp. 99–245. Marcel Dekker Inc., New York.

Hearn M.T.W. and Grego B. 1981. Organic solvent modifier effects in the separation of unprotected peptides by reversed-phase liquid chromatography. J. Chromatogr. 218: 497–507.

Hearn M.T.W. and Grego B. 1983a. High performance liquid chromatography of amino acids, peptides and proteins. XLVI. Selectivity effects of peptidic positional isomers and oligomers separated by reversed-phase high-performance liquid chromatography. J. Chromatogr. 266: 75–87.

Hearn M.T.W. and Grego B. 1983b. High performance liquid chromatography of amino acids, peptides and proteins. LIII. Evaluation of the effect of several stationary phase parameters on the chromatographic separation of polypeptides on alkylsilicas. J. Chromatogr. 282: 541–560.

Hearn M.T.W. and Grego B. 1983c. High-performance liquid chromatography of amino acids, peptides and proteins. XL. Further studies on the role of the organic modifier in reversed-phase high-performance liquid chromatography of polypeptides. Implications for gradient optimisation. J. Chromatogr. 255: 125–136.

Hearn M.T.W. and Zhao G.L. 1999. Investigations into the thermodynamics of polypeptide interaction with nonpolar ligands. Anal. Chem. 71: 4874–4885.

Hearn M.T.W., Keah H.H., Boysen R.I., Messana I., Misiti F., Rossetti D.V., Giardina B., and Castagnola M. 2000. Determination of biophysical parameters of polypeptide retro-inverso isomers and their analogues by capillary electrophoresis. Anal. Chem. 72: 1964–1972.

Herring S.W. and Enns R.K. 1983. Rapid purification of leukocyte interferons by high-performance liquid chromatography. J. Chromatogr. 266: 249–256.

Heukeshoven J. and Dernick R. 1985. Characterization of a solvent system for separation of water-insoluble poliovirus proteins by reversed-phase high-performance liquid chromatography. J. Chromatogr. 326: 91–95.

Horváth C., Melander W., and Molnár I. 1976. Solvophobic interactions in liquid chromatography with nonpolar stationary phases. J. Chromatogr. 125: 129–156.

_______. 1977a. Liquid chromatography of ionogenic substances with nonpolar stationary phases. Anal. Chem. 49: 142–154.

Horváth C., Melander W., Molnár I., and Molnár P. 1977b. Enhancement of retention by ion-pair formation in liquid chromatography with nonpolar stationary phases. Anal. Chem. 49: 2295–2305.

I T.O., Guhan S., Taksen K., Vavra M., Myers D., and Hearn M.T.W. 2002. Intelligent automation of HPLC method development using a real-time knowledge-based approach. J. Chromatogr. A (in press).

Ishii D., Asai K., Hibi K., Jonakuchi T., and Nagaya M. 1977. A study of micro-high-performance liquid chromatography. I. Development of technique for miniaturization high-performance liquid chromatography. J. Chromatogr. 144: 157–168.

Jeppsson J.O., Källman I., Lindgren G., and Fägerstam L.G. 1984. Hb-linköping (beta36 ProrarrThr): A new hemoglobin mutant characterized by reversed-phase high-performance liquid chromatography. J. Chromatogr. 297: 31–36.

Keah H.H., O'Bryan M., de Kretser D.M., and Hearn M.T.W. 2001a. Synthesis and application of peptide immunogens related to the sperm tail protein tpx-1, a member of the CRISP superfamily of proteins. J. Peptide Res 57: 1–10.

Keah H.H., Allen N., Clay R., Boysen R.I., Warner T., and Hearn M.T.W. 2001b. Total chemical synthesis of activin betaA[12-115] and related large loop polypeptides. Biopolym. Pept. Sci. 60: 279–289.

Kopaciewicz W. and Regnier F.E. 1983. A system for coupled multiple-column separation of proteins. Anal. Biochem. 129: 472–482.

Kucera P. 1980. Design and use of short microbore columns in liquid chromatography. J. Chromatogr. 198: 93–109.

Lankmayr E.P., Wegscheider W., and Budna K.W. 1989. Global optimization of HPLC separations. J. Liq. Chromatogr. 12: 35–58.

Lewis R.V., Fallon A., Stein S. Gibson K.D., and Udenfriend S. 1980. Supports for reverse-phase high-performance liquid chromatography of large proteins. Anal Biochem. 104: 153–159.

Mahoney W.C. and Hermodson M.A. 1980. Separation of large denatured peptides by reverse phase high performance liquid chromatography. Trifluoroacetic acid as a peptide solvent. J. Biol. Chem. 255: 11199–11203.

Mant C.T. and Hodges R.S. 1991a. The effects of anionic ion-pairing reagents on the peptide retention in reversed-phase chromatography. In High-performance liquid chromatography of peptides and proteins: Separation, analysis, and conformation (ed. C.T. Mant and R.S. Hodges), pp. 327–341. CRC Press, Boca Raton, Florida.

_______. 1991b. Requirements for peptide standards to monitor column performance and the effect of column dimensions, organic modifiers, and temperature in reversed-phase chromatography. In High-performance liquid chromatography of peptides and proteins: Separation, analysis, and conformation (ed. C.T. Mant and R.S. Hodges), pp. 289–295. CRC Press, Boca Raton, Florida.

Mant C.T., Kondejewski L.H., Cachia P.J., Monera O.D., and Hodges R.S. 1997. Analysis of synthetic peptides by high-performance liquid chromatography. Methods Enzymol. 289: 426–469.

Molnar I., Boysen R.I., and Erdmann V.A. 1989a. Reversed-phase high-performance liquid chromatography of Thermus aquaticus 50S and 30S ribosomal proteins. Chromatographia 28: 39–44.

Molnar I., Boysen R.I., and Jekow P. 1989b. Peak tracking in high-performance liquid chromatography based on normalized band areas. A ribosomal protein sample as an example. J. Chromatogr. 485: 569–579.

Mönch W. and Dehnen W. 1978. High-performance liquid chromatography of polypeptides and proteins on a reversed-phase support. J. Chromatogr. 147: 415–418.

Moritz R.L. and Simpson R.J. 1992a. Application of capillary reversed-phase high-performance liquid chromatography to high-sensitivity protein sequence analysis. J. Chromatogr. 599: 119–130.

_______. 1992b. Purification of proteins and peptides for sequence-analysis using microcolumn liquid-chromatography. J. Microcolumn. Sep. 4: 485–489.

Moritz R.L., Reid G.E., Ward L.D., and Simpson R.J. 1994. Capillary HPLC: A method for protein isolation and peptide mapping. Methods 6: 213–226.

Nice E.C. and O'Hare M.J. 1978. Selective effects of reversed-phase column packings in high-performance liquid chromatography of steroids. J. Chromatogr. 166: 263–267.

Nice E.C., Capp M., and O'Hare M.J. 1979. Use of hydrophobic interaction methods in the isolation of proteins from endocrine and paraendocrine tissues and cells by high performance liquid chromatography. J. Chromatogr. 185: 413–427.

Nice E.C., Grego B., and Simpson R.J. 1985. Application of short microbore HPLC guard columns for the preparation of samples for protein microsequencing. Biochem. Int. 11: 187–195.

Nice E.C., Lloyd C.J., and Burgess A.W. 1984. Role of short microbore high-performance liquid chromatography columns for protein separation and trace enrichment. J. Chromatogr. 296: 153–170.

Nick H.P, Wettenhall R.E., and Hearn M.T. 1985. Isolation of protein S6 from rat liver ribosomes by reversed-phase high-performance liquid chromatography. Anal Biochem. 148: 93–100.

Novotny M.V. and Ishii D., eds. 1985. Microcolumn separations: Columns, instrumentation and ancillary techniques. J. Chromatogr., vol. 30. Elsevier, The Netherlands.

O'Hare M.J., Capp M.W., Nice E.C., Cooke N.H., and Archer B.G. 1982. Factors influencing chromatography of proteins on short alkylsilane-bonded large pore-size silicas. Anal Biochem. 126: 17–28.

Pohl T. and Kamp R.M. 1987. Desalting and concentration of proteins in dilute solution using reversed-phase high-performance-liquid-chromatography. Anal. Biochem. 16: 388–391.

Purcell A.W., Aguilar M.I., and Hearn M.T.W. 1993. High-performance liquid chromatography of amino acids, peptides, and proteins. 123. Dynamics of peptides in reversed-phase high-performance liquid chromatography. Anal. Chem. 65: 3038–3047.

Purcell A.W., Zhao G.L., Aguilar M.I., and Hearn M.T.W. 1999. Comparison between the isocratic and gradient retention behaviour of polypeptides in reversed-phase liquid chromatographic environments. J. Chromatogr. 852: 43–57.

Putterman G.J., Spear M.B., Meade Cobun K.S., Widra M., and Hixson C.V. 1982. A rapid isolation of human chorionic gonadotropin and its subunits by reversed-phase high performance liquid chromatography. J. Liq. Chromatogr. 5: 715–730.

Quarry M.A., Grob R.L., and Snyder L.R. 1986. Prediction of precise isocratic retention data from two or more gradient elution runs. Analysis of some associated errors. Anal. Chem. 58: 907–917.

Rivier J. and McClintock R. 1989. Isolation and characterization of biologically active peptides and proteins using reversed-phase HPLC. In The use of HPLC in receptor biochemistry (ed. A.R. Kerlavage), pp. 77–103. A.R. Liss, New York.

Round A.J., Aguilar M.I., and Hearn M.T. 1994. High-performance liquid chromatography of amino acids, peptides and proteins. CXXXIII. Peak tracking of peptides in reversed-phase high-performance liquid chromatography. J. Chromatogr. A 661: 61–75.

Scott R.P.W. and Kucera P. 1979a. Mode of operation and performance characteristics of microbore columns for use in liquid chromatography. J. Chromatogr. 169: 51–72.

_______. 1979b. Use of microbore columns for the separation of substances of biological origin. J. Chromatogr. 185: 27–41.

Simpson R.J. and Nice E.C. 1987. The role of microbore HPLC in the purification of subnanomole amounts of polypeptides and proteins for gas-phase sequence analysis. In Methods in protein sequence analysis–1986 (ed. K. Walsh), pp. 213–228. Humana Press, Clifton, New Jersey.

_______. 1989. Strategies for the purification of subnanomole amounts of protein and polypeptides for microsequence analysis. In The use of HPLC in receptor biochemistry (ed. A.R. Kerlavage), pp. 201–244. A.R. Liss, New York.

Simpson R.J., Moritz R.L., Reid G.E., and Ward L.D. 1991. Current strategies for microscale purification of protein and peptides for sequence analysis. In Methods in protein sequence analysis (ed. H. Jornvall et al.), pp. 67–77. Birkhauser Verlag, Basel, Switzerland.

Simpson R.J., Moritz R.L., Begg G.S., Rubira M.R., and Nice E.C. 1989a. Micropreparative procedures for high sensitivity sequencing of peptides and proteins. Anal. Biochem. 177: 221–236.

Simpson R.J., Moritz R.L., Rubira M.R., Gorman J.J., and Van Snick J. 1989b. Complete amino acid sequence of a new murine T-cell growth factor P40. Eur. J. Biochem. 183: 715–722.

Sitaram B.R., Keah H.H., and Hearn M.T.W. 1999. Studies on the relationship between structure and electrophoretic mobility of alpha-helical and beta-sheet peptides using capillary zone electrophoresis. J. Chromatogr. 857: 263–273.

Skinner S.J.M., Grego B., Hearn M.T.W., and Liggins G.C. 1984. The separation of collagen alpha-chains by reversed-phase high-performance liquid chromatography. Comparison of column alkyl stationary phases and temperature effects. J. Chromatogr. 308: 111–119.

Snyder L.R. 1980. Gradient elution. In HPLC: Advances and perspectives (ed. C. Horvath), vol. 1, pp. 208–316. Academic Press, New York.

_______. 1990. Gradient elution separation of large biomolecules. In HPLC of biological macromolecules: Methods and applications (ed. K.M. Gooding and F.E. Regnier), pp. 231–259. Marcel Dekker, New York.

Snyder L.R., Stadalius M.A., and Quarry M.A. 1983. Gradient elution in reversed-phase HPLC separation of macromolecules. Anal. Chem. 55: 1413–1430.

Sottrup-Jensen L., Stepanik T.M., Jones C.M., Lonblad P.B., Kristensen T., and Wierzbicki D.M. 1984. Primary structure of human alpha 2-macroglobulin. I. Isolation of the 26 CNBr fragments, amino acid sequence of 13 small CNBr fragments, amino acid sequence of methionine-containing peptides, and alignment of all CNBr fragments. J. Biol. Chem. 259: 8293–8303.

Stadalius M.A., Gold H.S., and Snyder L.R. 1984. Optimization model of for the gradient elution separation of peptide mixtures by reversed-phase high performance liquid chromatography. Verification of retention relationships. J. Chromatogr. 296: 31–59.

Stanton P.G. and Hearn M.T. 1987. The iodination sites of bovine throtropin. J. Biol. Chem. 262: 1623–1632.

Stein S., Kenny C., Friesen H.J., Shively J., Del Valle U., and Pestka S. 1980. NH2-terminal amino acid sequence of human fibroblast interferon. Proc. Natl. Acad. Sci. 77: 5716–5719.

Strasters J.K., Billiet H.A.H., de Galan L., Vandeginste B.G.M., and Kateman G. 1989. Automated peak recognition from photodiode array spectra in liquid chromatography. J. Liq. Chromatogr. 12: 3–22.

Uyttenhove C., Simpson R.J., and Van Snick J. 1988. Functional and structural characterization of P40, a mouse glycoprotein with T-cell growth factor activity. Proc. Natl. Acad. Sci. 85: 6934–6938.

van der Zee R. and Welling G.W. 1982. Molecular sieving during reversed-phase high-performance liquid chromatography of proteins. J. Chromatogr. 244: 134–136.

van der Zee R., Welling-Wester S., and Welling G.W. 1983. Purification of detergent-extracted Sendai virus proteins by reversed-phase high-performance liquid chromatography. J. Chromatogr. 266: 577–584.

Walhagen K., Unger K.K., and Hearn M.T.W. 2000a. Capillary electroendoosmotic chromatography of peptides. J. Chromatogr. 887: 165–185.

_______. 2000b. Influence of temperature on the behaviour of small linear peptides in capillary electrochromatography. J. Chromatogr. 893: 401–409.

_______. 2001. Capillary electrochromatography analysis of hormonal cyclic and linear peptides. Anal. Chem. 73: 4924–4936.

Welinder K.G. 1988. Generation of peptides suitable for sequence analysis by proteolytic cleavage in reversed-phase high-performance liquid chromatography solvents. Anal. Biochem. 1: 54–64.

Wiktorowicz J.E., ed. 1992. Capillary electrophoresis. Methods, vol. 4, issue 3. Academic Press, New York.

Witting L.A., Gisch D.J., Ludwig R., and Eksteen R. 1984. Bonded-phase selection in the high-performance liquid chromatography of proteins. J. Chromatogr. 296: 97–105.

Wolfe R.A., Casey J., Familletti P.C., and Stein S. 1984. Isolation of proteins from crude mixtures with silica and silica-based adsorbents. J. Chromatogr. 296: 277–284.

Yang F.J. 1982. Fused-silica narrow-bore micro-particle-packed-column high-performance liquid chromatography. J. Chromatogr. 236: 265–277.

Further Reading

Frenz J., Hancock W.S., and Henzel W.J. 1990. Reversed phase chromatography in analytical biotechnology of proteins. In HPLC of biological macromolecules: Methods and applications (ed. K.M. Gooding and F.E. Regnier), pp. 145–177. Marcel Dekker, New York.

Hearn M.T.W. 1998. High-resolution reversed-phase chromatography. In Protein purification: Principles, high-resolution methods, and applications, 2nd edition (ed. J.-C. Janson and L. Rydén), pp. 239–282. Wiley-Liss, New York.

Mant C.T. and Hodges R.S., eds. 1991. High-performance liquid chromatography of peptides and proteins: Separation, analysis, and conformation. CRC Press, Boca Raton, Florida.

 
 
 

 
   
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