About the Book

Dynamic modulation of protein–protein interactions provides the physical basis for many biological signaling networks. Consequently, over the last several decades, much effort has been invested in defining and understanding these interactions. Knowledge of the pattern and regulation of discrete protein–protein interactions, and the larger networks that are built from them, tells us how living organisms function as homeostatic entities in the face of a changing environment and also how they develop and change their phenotypes over time or in response to specific triggers. In addition, characterization of protein–protein interactions informs us of the molecular basis of human disease and provides opportunities to intervene to prevent, detect, and treat disease. The goal of this book is to describe classic and cutting edge techniques to identify, characterize, and modulate simple and complex protein–protein interactions. This manual, which includes discussions and analyses that accompany detailed step-by-step protocols, is presented in eight sections. More…

About the Editors and Authors

Erica Golemis, Ph.D., is a Senior Member and Peter D. Adams, Ph.D., is a Member of the Faculty of Fox Chase Cancer Center in Philadelphia, Pennsylvania. More…

Review

[of Protein–Protein Interactions, first edition]

“Proteins control and mediate many of the biological activities of cells. Hence, to gain an understanding of cellular function, the function of every protein must be understood, both in isolation and in the context of other interacting proteins. This view was established many years ago, but developments in genomics have led several scientists to invert this statement: if we knew most or all protein–protein interactions in a cell, the functions of most proteins would become clearer. Recently, we have seen the development of large-scale biological experiments that are technology-driven rather than hypothesis-driven. And, it has become evident that one can easily forget the main goal of biological achievement—understanding mechanisms of life—while concentrating on high-throughput technologies to populate biological databases. …

Protein–Protein Interactions, edited by Erica Golemis, reconciles modern large-scale technological approaches with more classical approaches to understanding proteins. … More importantly, the book often shows how protein interaction measurements help the understanding of biological mechanisms. This book develops an appreciation of the wide range of techniques addressing protein–protein interaction issues, and why some are better suited than others to answer a specific biological question.

Pierre Legrain
Hybrigenics SA, VP Science & Technology, Paris, France
In Trends in Biotechnology, Vol. 20, No. 8, pp. 358–359, August 2002