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Protein–Protein Interactions: A Molecular Cloning Manual, Second Edition—Protocols [Supplementary material]

Chapter 11: Bacterial Two-Hybrid System for Studying and Modifying Protein–Protein Interactions

Chapter 14: Analytical Ultracentrifugation in the Study of Protein Self-Association and Heterogeneous Protein–Protein Interactions

Chapter 20: Advancement and Utilization of Biosensors for Protein Interaction Analysis and Proteomics

Chapter 35: Imaging Protein–Protein Interactions in Living Animals

Protein–Protein Interactions: A Molecular Cloning Manual, First Edition—Chapters

Sixteen chapters from the first edition were not included in the second edition because of space limitations, balanced with the need to accommodate new material. Many of these chapters remain relevant and interesting and are available here as pdfs.

Chapter 2: Signal Transduction and Mammalian Cell Growth: Problems and Paradigms

Chapter 3: Impact of Protein Interaction Technologies on Cancer Biology and Pharmacogenetics

Chapter 11: Detection of Homotypic Protein Interactions with Green Fluorescent Protein Proximity Imaging (GFP-PRIM)

Chapter 15: Analysis of Protein Interactions Using a Quartz Crystal Microbalance Biosensor

Chapter 16: Protease Footprinting

Chapter 17: Tandem Affinity Purification to Enhance Interacting Protein Identification

Chapter 18: Protein Purification by Inverse Transition Cycling

Chapter 19: Using In Vitro Expression Cloning to Identify Interacting Proteins

Chapter 20: Using Xenopus Egg Extracts to Modify Recombinant Proteins

Chapter 21: Using λ Repressor Fusions to Isolate and Characterize Self-assembling Domains

Chapter 23: Protein Interactions in Live Cells Monitored by β-Galactosidase Complementation

Chapter 24: Identification of Protein Single-chain Antibody Interactions In Vivo Using Two-hybrid Protocols

Chapter 28: Generation of Protein Fragment Libraries by Incremental Truncation

Chapter 29: Catalytic Antibodies: New Characters in the Protein Repertoire

Chapter 32: Protein Bundling to Enhance the Detection of Protein–Protein Interactions

Chapter 35: Modulating Protein–Protein Interactions to Develop New Therapeutic Approaches

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