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Chapter 1: Introduction to Proteomics
Chapter 2: One–dimensional Polyacrylamide Gel Electrophoresis
Chapter 3: Preparing Cellular and Subcellular Extracts
Chapter 4: Preparative Two–dimensional Gel Electrophoresis with Immobilized pH Gradients
Chapter 5: Reversed–phase High–performance Liquid Chromatography
Chapter 6: Amino– and Carboxy– terminal Sequence Analysis
Chapter 7: Peptide Mapping and Sequence Analysis of Gel–resolved Proteins
Chapter 8: The Use of Mass Spectrometry in Proteomics
Chapter 9: Proteomic Methods for Phosphorylation Site Mapping
Chapter 10: Characterization of Protein Complexes
Chapter 11: Making Sense of Proteomics: Using Bioinformatics to Discover a Protein's Structure, Functions, and Interactions
Appendices 1 and 2
WWW Resources
 
Figures

 
Tables

Temperature conversion
Pressure conversion
Tubing internal diameters and volumes
Moles/weight conversion
Some properties of posttranslational derivatives
Mass changes due to some posttranslational modifications of peptides and proteins
The masses and compositions of commonly occurring amino acid residues
Properties of amino acids
Proteins used as molecular-weight standards
Proteases
Protease inhibitors
Structure and classification of detergents
The avoidance of proteolysis during sample preparation
Nonionic detergents
Ionic detergents
Zwitterionic detergents
 
Protocols

Analysis of Carbohydrate from Glycoproteins
Sample Preparation for High-resolution Two-dimensional Electrophoresis by Isoelectric Fractionation in an MCE
2D Gel Troubleshooting Guide
HPLC Troubleshooting
 Contents
 1. Introduction
 2. Abnormal Pressure
 3. Leaks
 4. Problems with the Chromatogram
 5. Problems with the Injector
 6. Problems Detected by Smell, Sight, or Sound
 7. Key Problem Areas and Preventive Maintenance
 
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 Chapter 1: Introduction to Proteomics
 Chapter 2: One–dimensional Polyacrylamide Gel Electrophoresis
 Chapter 3: Preparing Cellular and Subcellular Extracts
 Chapter 5: Reversed–phase High–performance Liquid Chromatography
 Chapter 6: Amino– and Carboxy– terminal Sequence Analysis
 Chapter 7: Peptide Mapping and Sequence Analysis of Gel–resolved Proteins
 Chapter 8: The Use of Mass Spectrometry in Proteomics
 Chapter 9: Proteomic Methods for Phosphorylation Site Mapping
 Chapter 10: Characterization of Protein Complexes
 Chapter 11: Making Sense of Proteomics: Using Bioinformatics to Discover a Protein's Structure, Functions, and Interactions
 Appendices 1 and 2
 
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